In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

Background: Monovarietal cultivation of cotton plant allowed the genetic erosion for traditional varieties that proceed essential traits mainly involved in breeding. These need to be preserved future used. This study aims evaluate effect gibberellic acid on seed germination and Benzylaminopurin (BAP), Kinetin (KIN), α-naphthalene acetic (NAA) activated charcoal (CA) seedlings growth obtained from different type explants.
 Methodology: The seeds three improved (KET782, ANG956, OKP768) five local (Q62, Q64, Q85, Q88, Q92) were germinated with concentration (GA3). Different explants collected plantlets cultivated culture media containing plants regulators concentration.
 Results: We found Gibberellic Acid activity significantly varied according variety GA3 medium. 63.33% rate OKP768 was medium 0.5 mg/L while 1 mg/l gave highest Q85 (75%), Q64 (69.17%), ANG956 (40.83%). regeneration within 100%of axillary nodes regenerated Q62; Q92 60% zygotic embryos OKP768. Moreover, supplemented (1mg/l) + NAA (1 mg/l) induced greatest number roots (2.75 roots/plantlet) varieties. With (10 g/l), achieved better performance respective length average 3.4 cm/plantlet 2.1 cm/plantlet. at 10 g/l highly influenced an 7.7cm variety.
 Implication: protocol established during this will useful vitro conservation